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From Plants to Sequences: A Six-Week College Biology Lab Course
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Event Recorded on:
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Friday, 16 May 2008, 10:00 AM - 11:00 AM
(GMT -07:00) Pacific Time (US & Canada); Tijuana
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Abstract:
In this FREE web conference, we will share the Cloning and Sequencing Explorer Series for student research developed in collaboration with Bio-Rad Laboratories (available Fall 2008).
Gene-cloning techniques are a common topic in most molecular biology courses, but providing students with hands-on experience is more challenging. The objective of this project is to have students isolate, sequence and characterize a pivotal gene of glycolysis (Glyceraldehyde 3-Phosphate Dehydrogenase; GAPDH) from a plant species, or cultivar, not previously published. In addition to wet-lab techniques (e.g. DNA extraction, PCR, ligation, transformation, restriction digest), students gain experience with bioinformatics analysis of their unique clones. This includes BLAST, identification of introns/exons, contig construction, phylogenetics, and acquaintance with different bioinformatics databases. These results are publishable in the NCBI GenBank.
Presenters:
Dr. David Robinson
Dr. Joann Lau
Department of Biology, Bellarmine University,
2001 Newburg Road, Louisville, KY 40205
*Note on system compatibility:
This webinar will be conducted using internet audio with the Centra Client software. It is advised that you do a system check to make sure that your system is compatible with the client software.
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