Exploring One-Shot Kinetics and Small Molecule Analysis
Using the ProteOn™ XPR36 Protein Interaction Array System

Event Recorded on:
Wednesday, 15 November 2006, 1:00 PM - 3:00 PM (GMT -07:00) Pacific Time (US & Canada); Tijuana


A ProteOn XPR36 biosensor was used to characterize a set of small molecule/enzyme interactions. Using one injection with the ProteOn's crisscross flow system, we collected response data for six different concentrations of each analyte over six different target protein surfaces. This ‘one-shot' approach to kinetic analysis improves throughput while generating high-quality data. Our results illustrate the potential of this parallel-processing biosensor to increase the speed of kinetic analysis in drug discovery and expand the applications of real-time protein interaction arrays.


David Myszka
Dr. David Myszka is the founder and director of the Center for Biomolecular Interaction Analysis at the University of Utah.



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